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      當前位置:科學網首頁 > 基金首頁 > 生物絮團中聚β-羥基丁酸酯(PHB)對梭魚免疫調控的研究

      國家自然科學基金項目查詢

      生物絮團中聚β-羥基丁酸酯(PHB)對梭魚免疫調控的研究

      批準號31602179 學科分類魚類養殖學 ( C190501 )
      項目負責人喬幗 負責人職稱副教授 依托單位鹽城工學院
      資助金額19.00
      萬元
      項目類別青年科學基金項目 研究期限2017 年 01 月 01 日 至
      2019 年 12 月 31 日
      中文主題詞魚類;健康養殖;聚β-羥基丁酸酯(PHB);免疫;調節
      英文主題詞Fish;Green culture;Poly-β-hydroxybutyrate (PHB);Immunity;Regulation

      摘要

      中文摘要 生物絮團技術是一種新興的零排放生態養殖技術,在水產養殖中具有廣闊的應用前景。聚β-羥基丁酸酯 (poly-β-hydroxybutyrate,PHB) 是生物絮團中的重要活性成分,可提高養殖動物免疫力,但與其作用相關的調控機制尚不清楚。項目組已從生物絮團中分離PHB并構建了魚類最佳投喂模式。本項目擬以江蘇重要養殖魚類—梭魚為養殖對象,研究PHB的免疫調控機制。主要內容:①采用生化技術分析PHB對梭魚免疫相關酶酶活的影響;②采用RNA-seq技術構建PHB刺激后mRNA表達譜,篩選差異表達基因,分析其特征和調控網絡;③采用qRT-PCR分析差異基因的時空表達,揭示PHB的空間作用靶點和影響度;④分子克隆靶基因啟動子序列,分析啟動子功能。本研究將從酶和基因水平揭示PHB對魚類免疫的調控機制,有望為新型養殖技術的推廣應用提供理論基礎和技術支撐。因此,本項目具有重要的科學意義和較高的應用價值。
      英文摘要 Biofloc technology is a zero-water exchange, environment-friendly farming system and it has been widely used in aquaculture since it could enhance the fish growth and immunity. Poly-β-hydroxybutyrate (PHB) in bioflocs is an importantly activated compound and it could stimulate the immune response of aquatic species, especially for fish. However, there is little known about its molecular mechanisms in fish upon PHB stimulation. In our laboratory, we have extracted the PHB from bioflocs and established PHB feeding methods. In this proposal, we will select the soiny mullet (Liza haematocheila), an important aquaculture species as model system to investigate the molecular mechanisms how PHB could enhance the immunity. The main research subjects include: (1) with biochemical analytic methods to analyze the immune-related enzymatic changes upon PHB treatment; (2) applying RNA-seq technology to comparatively analyze the transcriptomic profile in L. haematocheila upon PHB stimulation, and unigenes functional annotation and pathway will be predicted through bioinformatics methods; (3) the crucial genes related to immunity will be screened and their spatial differential expression status will be assayed using qRT-PCR technology, so as to reveal their potential function; Finally,(4) the promoters of target genes will be cloned and sequenced, and then plasmid without promoters will be constructed, with which how PHB could affect the activity of promoters will be assayed. Therefore, this study will elucidate the enzymatic and molecular mechanisms of L. haematocheila against PHB stimulation at both transcriptional level and post-transcriptional level. Thus, our study will provide a comprehensive understanding of the molecular mechanisms in fish with biofloc systems and it embraces great significances in both basic theory and practical application in aquaculture.
      結題摘要

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